黄振华,梁炜,潘磊,张鹏,付敏,钱晖,许文荣,蒋鹏程,张徐.胃癌患者血清LINC00978表达及其临床意义[J].临床检验杂志,2017,(8):575-578
胃癌患者血清LINC00978表达及其临床意义
Expression of serum LINC00978 in gastric cancer patients and its clinical significance
投稿时间:2017-05-22  
DOI:
中文关键词:  胃癌  LINC00978  血清  生物学标志物
英文关键词:gastric cancer  LINC00978  serum  biomarker
基金项目:国家自然科学基金面上项目(81572075, 81672416);江苏省自然科学基金面上项目(BK20141303);江苏省重点研发计划(社会发展)项目(BE2015667);镇江市重点研发计划(社会发展)项目(SH2015034);江苏省“青蓝工程”,江苏省“333工程”。
作者单位
黄振华 江苏大学医学院江苏省检验医学重点实验室江苏镇江 212013/江苏大学附属人民医院普外科江苏镇江 212002 
梁炜 江苏大学医学院江苏省检验医学重点实验室江苏镇江 212013 
潘磊 江苏大学医学院江苏省检验医学重点实验室江苏镇江 212013/江苏大学附属人民医院普外科江苏镇江 212002 
张鹏 江苏大学医学院江苏省检验医学重点实验室江苏镇江 212013 
付敏 江苏大学附属人民医院普外科江苏镇江 212002 
钱晖 江苏大学医学院江苏省检验医学重点实验室江苏镇江 212013 
许文荣 江苏大学医学院江苏省检验医学重点实验室江苏镇江 212013 
蒋鹏程 江苏大学附属人民医院普外科江苏镇江 212002 
张徐 江苏大学医学院江苏省检验医学重点实验室江苏镇江 212013 
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中文摘要:
      摘要:目的:检测胃癌患者血清中LINC00978〖表达水平,评估其表达水平与临床病理参数的关系,并探讨其临床诊断价值。 方法:实时荧光定量RT-PCR检测LINC00978在胃癌细胞、体检健康者、胃良性疾病及胃癌患者血清中的表达水平,分析其与胃癌患者临床病理参数的相关性,并用ROC曲线分析其诊断效能。 结果:LINC00978在人胃癌细胞系MGC-803中的表达水平(18.88±1.15)明显高于胃黏膜上皮细胞(1.00±0.03),差异有统计学意义(t=-21.926,P<0.05)。LINC00978在胃癌患者血清中的平均含量[3.525(1.385,8.954)]较胃良性疾病患者[0.419(0.258,1.369)]和体检健康者[0.814(0.351,2.510)]均明显升高(Z值分别为-4.834和-4.686;P均<0.01),且与淋巴结转移、浸润深度和TNM分期相关(r分别为0.448、0.369和0.383,P均<0.01);胃癌患者术后血清中LINC00978的表达水平[0.17(0.15,0.39)]较术前[0.98(0.59,1.61)]明显降低(Z=-5.731,P<0.01)。与体检健康者相比,LINC00978在胃良性疾病患者血清中表达差异无统计学意义(Z=-1.693,P>0.05)。胃癌患者血清LINC00978的ROC曲线下面积(AUCROC)为0.807,95%可信区间(CI)为0.723~0.882,当cut off值为1.806时,其诊断胃癌的敏感性为71.4%,特异性为75.9%。 结论:LINC00978在胃癌患者血清中呈高表达,且与淋巴结转移、浸润深度和TNM分期相关,有望成为潜在的胃癌诊断的生物学标志物。
英文摘要:
      Abstract: Objective:To investigate the expression levels of serum LINC00978 in gastric cancer patients and its correlation with clinicopathological characteristics, and evaluate its clinical diagnostic value. Methods:The expression levels of LINC00978 in gastric cancer cells and serum samples from the patients with gastric cancer or gastric benign diseases and healthy controls were detected by real-time qRT-PCR, and its correlations with clinicopathological parameters were analyzed. The diagnostic efficacy of serum LINC00978 was evaluated by the receiver operating characteristic (ROC) curve. Results:The expression levels of LINC00978 in MGC-803 cells were significantly higher than that in GES 1 cells (18.88±1.15 vs 1.00±0.03, t=-21.926, P<0.05). The expression levels (median[P25, P75]) of serum LINC00978 in gastric cancer patients (3.525[1.385, 8.954]) were significantly higher than those in the patients with gastric benign diseases (0.419[0.258, 1.369], Z=-4.834, P<0.01) and healthy controls (0.814[0.351, 2.510], Z=-4.686, P<0.01). The expression levels of serum LINC00978 in gastric cancer patients were significantly related to lymphatic metastasis (r=0.448, P<0.01), invasive depth (r=0.369, P<0.01) and TNM stage (r=0.383, P<0.01), and those after operation significantly lower than before operation (0.17[0.15,0.39] vs 0.98[0.59,1.61], Z=-5.731,P<0.01). There was no significant difference in serum LINC00978 levels between the patients with gastric benign diseases and healthy controls (Z=-1.693, P>0.05). The area under the ROC curve (AUCROC) and 95% confidence interval (CI) of serum LINC00978 levels in the diagnosis of gastric cancer were 0.807 and 0.732 0.882, respectively. When the cutoff value was 1.806, the sensitivity and specificity for the diagnosis of gastric cancer were 71.4% and 75.9%, respectively. Conclusion:The expression levels of serum LINC00978 in gastric cancer patients increase and are related to lymphatic metastasis, invasive depth and TNM stage, which may be served as a novel biomarker for the diagnosis of gastric cancer.
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