Abstract: Objective：To establish a method of gold nanoprobebased solution hybridization (GNBSH) to detect nucleic acid sequencebased amplification (NASBA) products for the rapid diagnosis of invasive aspergillosis (IA). Methods：The Aspergillus specific 18S rRNA was amplified by NASBA and then the amplified products were hybridized with the gold nanoprobes which were modified with thiol compounds at the 5′ end. Serum samples from 106 patients, including 14 with a definite IA, 32 with suspected IA and 60 without IA, were detected by the established method, and the obtained results were compared with that of galactomannan (GM) test to evaluate its accuracy. Results：The gold nanoprobes only hybridized with Aspergillus NASBA products but not other nonAspergillus strains. The sensitivity, specificity and the area under the ROC curve (AUC^ROC) of the established GNBSH method for detecting 106 clinical samples were 82.61% (38/46), 81.67% (49/60) and 0.890, respectively. The sensitivity, specificity and AUC^ROC of GM test were 56.52% (26/46), 83.33% (50/60) and 0.723, respectively. Conclusion：The established GNBSH method to detect Aspergillus NASBA products has high sensitivity and specificity and simple operation, which may be used to detect the infection of Aspergillus by clinical laboratories.