陆攀,宋佳希,王锋,闫静,万淑君,汪俊军.血清miR-216a检测在急性胰腺炎诊断及预后监测中的价值[J].临床检验杂志,2017,(8):579-582
血清miR-216a检测在急性胰腺炎诊断及预后监测中的价值
Clinical significance of serum miR-216a in diagnosis and prognosis monitoring of patients with acute pancreatitis
投稿时间:2017-06-02  
DOI:
中文关键词:  急性胰腺炎  miR-216a  生物学标志物  预后监测
英文关键词:acute pancreatitis  miR-216a  biomarkers  prognosis monitoring
基金项目:国家自然科学基金(81572073;81572074)。
作者单位
陆攀 南京大学医学院附属金陵医院临床检验科南京 210002 
宋佳希 南京大学医学院附属金陵医院临床检验科南京 210002 
王锋 南京大学医学院附属金陵医院临床检验科南京 210002 
闫静 南京大学医学院附属金陵医院临床检验科南京 210002 
万淑君 南京大学医学院附属金陵医院临床检验科南京 210002 
汪俊军 南京大学医学院附属金陵医院临床检验科南京 210002 
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中文摘要:
      摘要:目的: 检测急性胰腺炎(AP)患者血清miR-216a的表达水平,探讨其作为AP诊断及预后监测指标的临床应用价值。 方法:实时荧光定量PCR(qRT-PCR)检测80例轻症急性胰腺炎(MAP)、80例重症急性胰腺炎(SAP)患者及74例健康人对照血清miR-216a的表达水平。全自动生化免疫分析仪检测各组淀粉酶(AMY)、脂肪酶(LPS)、Ca2+、葡萄糖(Glu)、红细胞比容(HCT)、三酰甘油(TG)、总胆固醇(TC)和C反应蛋白(CRP)等指标。 ROC曲线及相关性分析评估miR 216a对AP患者的临床应用价值。 结果:与健康人对照组[(11.12×10-5(5.83×10-5,19.12×10-5)]相比,AP患者血清miR-216a的水平 [38.49×10-5(24.05×10-5,62.02×10-5)]明显升高(U=-9.10,P<0.01)。但MAP与SAP患者血清miR-216a水平[分别为36.46×10-5(22.29×10-5,55.80×10-5),40.44×10-5(25.84×10-5,65.48×10-5)]差异无统计学意义(U=-0.96,P>0.05)。miR--216a用于鉴别对照组和AP患者的ROC曲线下面积(AUCROC)为0.870(95%CI:0.825~0.915),cut off值为0.61;用于鉴别对照组和MAP患者的AUCROC为0.865(95%CI:0.808~0.921),cut off值为0.59;用于鉴别对照组与SAP患者的AUCROC为0.876 (95%CI:0.822~0.930),cut off值为0.66。AP患者治疗好转后,其血清miR-216a的表达水平由[41.88×10-5(24.24×10-5,64.44×10-5)]下降至[20.58×10-5(11.01×10-5,41.91×10-5)],差异有统计学意义(U=5.24,P<0.01)。相关性分析结果显示,AP患者血清miR-216a的表达水平与CRP呈正相关(r=0.215 P=0.006)。 结论: AP患者血清miR 216a水平明显升高,可用于AP辅助诊断及预后监测。
英文摘要:
      Methods:Serum miR-216a levels were determined by quantitative reverse transcription PCR (qRT-PCR) assay among 80 mild acute pancreatitis (MAP) patients, 80 severe acute pancreatitis (SAP) patients and 74 healthy controls. And amylase (AMY), lipase (LPS), Ca2+, glucose (Glu), hematocrit (HCT), triglyceride (TG), total cholesterol (TC) and C reactive protein (CRP) were measured by biochemical analyzer. The clinical usefulness of miR-216a for AP patients was assessed by ROC curve analysis and correlation analysis. Results: Compared with healthy controls (11.12×10-5[5.83×10-5,19.12×10-5], the serum miR-216a levels were significantly increased in AP patients(38.49×10-5[24.05×10-5,62.02×10-5], (U=-9.10,P<0.01)。The serum miR-216a levels in MAP and SAP patients were (36.46×10-5[22.29×10-5,55.80×10-5]vs 40.44×10-5[25.84×10-5,65.48×10-5]),there was no significant difference between MAP and SAP patients(U-0.96,P>0.05). The areas under ROC curve (AUC) of miR-216a for differential healthy controls and AP patients was 0.870 (95%CI:0.825 0.915), cut off value is 0.61. AUCROC of miR-216a for differential healthy controls and MAP patients was 0.865 (95%CI: 0.808 0.921), cut off value is 0.59. And AUC of miR-216a for differential healthy controls and SAP patients was 0.876 (95%CI:0.822 0.930), cut off value is 0.66. Moreover, after the clinical improvement of the patients, the levels of serum miR 216a were significantly lowered from (41.88×10-5[24.24×10-5,64.44×10-5]) to (20.58×10-5[11.01×10-5,41.91×10-5]), the differences was significant(U=5.24,P<0.01). Correlation analysis showed that miR-216a was positively correlated with CRP (r=0.215,P=0.006) in AP patients. Conclusion: The levels of miR-216a in serum of AP patients were increased, which is a potential biomarker for the diagnosis and prognosis monitoring of AP.
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