Objective To understand the genotype distribution and antibiotic resistance of CRE in our hospital. Methods 613 clinical isolates of CRE in our hospital were collected, and then routine drug sensitivity tests were carried out, and the results of drug sensitivity were analyzed. Then, 68 CRE strains were randomly selected for PCR amplification to detect the BLA KPC, BLA IMP-1, BLA VIM-1, BLA OXA-48 and Bla NDM-1 genes of carbapenems. The final PCR positive results were sequenced by DNA, and the BLAST alignment was used to determine the genotype of CRE. Result 613 strains of CRE were as follows: Klebsiella pneumoniae 548 strains, 36 strains of Escherichia coli, 19 strains of Enterobacter cloacae, Citrobacter freundii 5 strains, 3 strains of Enterobacter aerogenes, Proteus mirabilis 2 strains. The results of PCR amplification and DNA sequencing were as follows: 58 strains were bla KPC-2, 5 strains were bla NDM-1 and 5 strains were bla IMP-1, and Bla VIM-2 and Bla OXA-48 were not detected. Conclusions The number of CRE is increasing year by year, and it is multidrug resistant to clinical antibiotics, and Bla KPC-2 is the main type of CRE . Medical institutions should strengthen the sense of hospital monitoring to prevent and control the spread and spread of CRE strains.