Objective: To investigate the clinical distribution, drug resistance and main genotypes of carbapenemase of Carbapenem- resistant Enterobacteriaceae(CRE )in Suqian area. Methods: 52 unrepeated clinical CRE isolates were collected beteen January and December 2016 from three hospitals in Suqian. Antimicrobial susceptibility testing was carried out using disk diffusion and ertapenem, imipenem, meropenem, tigecycline were conducted to determine minimum inhibitory concentrations(MICs) by E-test method. Carbapenemase genes were detected by Polymerase chain reaction(PCR). Results:CRE strains were isolated from various clinical specimens,of which the top two were sputum(38.5%) and urine(28.8%). SE. coli was the most common bacteria,followed by K. pneumoniaeS andSE. cloacae. Most isolates had high levels carbapenem resistance(MICs>32㎎/L)and the isolates carried non- carbapenase gene had higher MICs of ertapenem than of imipenem and meropenem. The resistance rate of CRE isolates to most antimicrobial agents was >90% except amikacin(40.4%),minocycline(30.8%),tigecycline(0%). The carbapenemaseSgene was present in 94.2% of the isolates,with a majority of genes being bla<sub>NDM-1</sub> (79.6%) and bla<sub>KPC</sub> (22.4%).Most of the bla<sub>NDM-1</sub>-producing isolates were E. coli(66.7%), K. pneumoniaeS(15.4%), E. cloacae(10.3%).All of the isolates carried bla<sub>KPC </sub>were K. pneumoniae except 1 E. coli to harbour both bla<sub>NDM-1</sub> and bla<sub>KPC</sub>. Conclusion: The CRE strains in suqian were mainly E. coli and the antibiotic resistance is very high. Production of carbapenemase can be the main factor contributing to carbapenem resistance ,among which bla<sub>NDM-1</sub> were the major types. High attention should be paid to monitoring CRE isolates and it is necessary to put appropriate infection control measures to prevent the spread of these bacteria.