Carbapenem-Resistant Enterobacteriaceae (CRE) has rapidly spread globally in the past decade, which has brought great challenges to global human health. And the plasmid-mediated horizontal propagation of carbapenemase genes is the main reason for sharply increasing of CRE, therefore, timely and accurate detection of CRE, especially the carbapenemase producing CRE, is very important for clinical treatment guidance and infection prevention and control. A number of rapid detection methods including phenotypes and genotypes detection of CRE have been developed and are expected to be used in clinical microbiology laboratories. The clinical treatment options for CRE infection are very limited, and a variety of active antibacterial drugs are commonly used in combination. At present, new drugs with improved activity and safety are still in the late stage of clinical development.