Abstract:Abstract Objective To explore the epidemiological distribution characteristics of glucose-6-phosphate dehydrogenase(G6PD), incidence of G6PD deficiency in neonates, and to determine its cutoff values. Methods All newborns (n=1.44million) born in 10 districts of Zhejiang Province from March 1, 2015 to September 31, 2017 were included in this study. Fluorescence analysis was used to determine the G6PD activity of dried blood spots and the direct ratio method of G6PD/6PGD was used to confirm the diagnosis of positive screening recalls. The results were analyzed by using nonparametric and chi-square tests. Results Significant difference was found in G6PD level among groups of different genders, gestational age, birth weight, blood sampling age, blood sampling season and districts(P<0.01). The male incidence of G6PD deficiency was significantly higher than female incidence. In different regions of Zhejiang province, the prevalence was highest in Lishui (0.38%) and lowest in Zhoushan (0.11%), basically showed a trend of high in the south and low in the north. .When the cut - off value of G6PD activity ranged from 2.60 U/g Hb to 2.80 U/g Hb , the sensitivity of G6PD deficiency screening for male and female newborns was 100%,and the correct index was the highest (about 0.99).Conclusions The G6PD level is affected by groups, time and districts. The incidence of G6PD deficiency was influenced by gender and different regions. The male and female cutoff values were initially set at 2.60 U/g Hb and 2.80 U/g Hb, respectively.