CD39+CD73+B细胞亚群在原发性胆汁性胆管炎患者中的变化及其临床价值分析
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江苏省青年医学人才培养项目(QNRC2016214);苏州市重大疾病、传染病预防和控制关键技术项目(GWZX201604);苏州市科教兴卫青年项目(kjxw2014046);江苏大学2018年度临床医学科技发展基金(JLY20180023);常熟市卫计委科技计划项目(csws201714)


Changes of CD39+CD73+ B cell subsets in patients with primary biliary cholangitis and their clinical value
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    摘要:

    摘要:目的:分析原发性胆汁性胆管炎(PBC)患者外周血CD39+CD73+B细胞亚群在B细胞中所占比例的变化,及其与PBC的临床指标间的关系。 方法:收集江苏苏州吴江区和常熟地区PBC患者60例,健康人对照者40例,以流式细胞术分析所有研究对象外周血CD39+CD73+B细胞在B细胞中的比例,多重微珠流式荧光法定量分析PBC患者抗线粒体抗体M2(AMA-M2)水平,全自动生化分析仪检测肝功能各项指标。统计分析CD39+CD73+B细胞比例的组间差异及其与自身抗体和肝脏酶指标之间的关系。 结果:PBC患者CD39+CD73+B细胞的比例较对照组显著降低[55.6%(44.9%,60.5%) vs 73.0% (69.8%,74.9%),P<0.001]。PBC组CD39+CD73+B细胞比例与AMA-M2呈明显的负相关关系(r=-0.349,P=0.027)。抗糖蛋白210抗体(anti-GP210)阳性患者CD39+CD73+B细胞比例较anti-GP210阴性患者明显降低[44.6%(38.2%, 49.9%) vs 58.7%(49.8%, 63.8%), P<0.001],抗核蛋体100抗体(anti-SP100)阳性和阴性患者间CD39+CD73+B细胞比例差异无统计学意义[58.6%(47.9%, 62.7%) vs 51.6%(44.7%, 60.4%), P=0.292]。 结论:PBC患者外周血CD39+CD73+B细胞比例显著降低,且与特异性自身抗体存在显著相关性,提示该群B细胞在PBC发病过程中可能发挥重要作用。

    Abstract:

    Abstract: Objective: To investigate the change of the proportion of CD39+CD73+ B cell subsets in B cells in peripheral blood of patients with primary biliary cholangitis (PBC) and its correlation with clinical indexes of PBC. Methods: Sixty PBC patients and 40 healthy controls from Wujiang and Changshu Districts of Suzhou were collected. The proportion of CD39+CD73+ B cell subsets in B cells in peripheral blood of all subjects was analyzed by flow cytometry. The levels of serum anti-mitochondrial antibody M2 (AMA-M2) in PBC patients were quantitatively analyzed by the automated multiplexed bead-based assay. The liver function indexes were detected by an automatic biochemical analyzer. The differences of CD39+CD73+ B cell subsets between groups and their correlations with autoantibodies and liver enzymes were analyzed. Results: The proportion [median (P25, P75)] of CD39+CD73+ B cell subsets in PBC patients was significantly lower than that in healthy controls [55.6%(44.9%, 60.5%) vs 73.0%(69.8%, 74.9%), P<0.001]. The proportions of CD39+CD73+ B cell subsets in PBC patients were significantly and negatively correlated with serum AMA-M2 levels (r=-0.349, P=0.027). The proportions of CD39+CD73+ B cell subsets in PBC patients with positive anti-glycoprotein 210 antibody (anti-GP210) were significantly lower than those with negative anti-GP210 [44.6%(38.2%, 49.9%) vs 58.7%(49.8%, 63.8%), P<0.001]. There was no significant difference in the proportion of CD39+CD73+ B cell subsets between the PBC patients with positive anti-nuclear body protein SP100 antibody (anti-SP100) and negative anti-SP100 [58.6%(47.9%, 62.7%) vs 51.6%(44.7%, 60.4%), P=0.292]. Conclusion: The proportion of CD39+CD73+ B cell subsets in peripheral blood of PBC patients is significantly reduced and correlated with specific autoantibodies, suggesting that these B cell subsets may play an important role in the pathogenesis of PBC.

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杨鸿林,蒋廷旺,龚燕萍,沈昊,钱靖,钮丽萍,朱安渡,钱雄杰. CD39+CD73+B细胞亚群在原发性胆汁性胆管炎患者中的变化及其临床价值分析[J].临床检验杂志,2020,(1):25-28

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  • 收稿日期:2019-05-31
  • 最后修改日期:2019-10-12
  • 录用日期:2020-02-03
  • 在线发布日期: 2020-07-24
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