| Abstract：Objective To investigate the drug resistance , genetic feature and genetic relationship of Acinetobacter baumannii(AB) isolated successively in pediatric patients with pneumonia. Methods 28 strains of AB were collected and isolated from the sputum culture of children with pneumonia, and were undertaken identification of bacteria and susceptibility test by Vitek-2 Compact automicroscan using GNI and GNS cards. Drug resistance genes ,integrons and transposons were analyzed by polymerase chain reaction (PCR). Results 4 of 28 strains of AB showed multidrug resistance, the positive rate was 14.29%. Beta-lactamase gene blaTEM, AmpC enzyme gene blaADC, modification gene of aminoglycosides aac(3)-II, aac(6’)-Ib, ant(3″)-I and aac(6″ )-II, integron genetic marker qacE△1-sul1, IntI1, and transposon genetic marker merA were all detected. Among them, 4 strains of blaTEM（14.29%）,17 strains of blaADC（60.71%）,1 strain of aac(3)-II（3.57%），1 strain of aac(6′)-Ib（3.57%），6 strains of ant(3″)-I（10.71%）, 2 strains of aac(6″)-II（7.14%）,11 strains of qacE△1-sul1（39.29%）,4 strains of IntI1（14.29%）and 3 strains of merA（10.71%） were discovered，the rest of beta-lactamase gene, metallo beta-lactamase gene, carbopenems gene, AmpC gene, modification gene of aminoglycosides, transposon genetic markers showed negative. This indicated that clone transmission phenomenon was existing in this group. Conclusions Comparing with adults, The types of drug resistance genes carried in Acinetobacter baumannii(AB) isolated successively in pediatric patients were less, and positive rates were lower, but clone transmission phenomenon was existing in this group, we should be on guard against outbreak epidemic induced by nosocomial infection.