儿童鲍曼不动杆菌相关耐药基因及亲缘性研究
The research on correlated drug resistance genes and the genetic relationship of Acinetobacter baumannii isolated in children
投稿时间:2010-04-15  修订日期:2010-09-15
DOI:
中文关键词:  儿童  肺炎  鲍曼不动杆菌  耐药基因  亲缘性
英文关键词:children  pneumonia  Acinetobacter baumannii  drug resistance genes  genetic relationship
基金项目:深圳市医学重点学科建设资助(2005C03)
作者单位E-mail
赵瑞珍 深圳市儿童医院 zhaoruizhen@126.com 
郑跃杰 深圳市儿童医院  
陈乾 深圳市儿童医院  
邓继岿 深圳市儿童医院  
金辉 东南大学公共卫生学院  
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中文摘要:
      摘要:目的 研究来自肺炎患儿的鲍曼不动杆菌连续分离株耐药性与遗传学特征及菌株的亲缘性。方法 收集自肺炎患儿痰标本分离的鲍曼不动杆菌(AB) 28株,用Vitek-2 Compact微生物鉴定仪GNI和GNS卡进行细菌鉴定和药敏试验;用PCR检测耐药基因和整合子、转座子。结果 28株AB菌有4株(14.3%)呈多重耐药性。β-内酰胺酶基因中的blaTEM阳性4株(14.3%);AmpC酶基因中的blaADC阳性17株(60.7%);氨基糖苷类修饰酶基因中的aac(3)-II阳性1株(3.6%)、aac(6’)-Ib阳性1株(3.6%)、ant(3’’)-I 阳性3株(10.7%)、aac(6’’)-II 阳性2株(7.1%);整合子遗传标记中的qacE△1- sul1阳性11株(39.3%)、IntI1阳性4株(14.3%);转座子遗传标记中的merA阳性3株(10.7%)。其余β-内酰胺酶基因、金属β-内酰胺酶基因、碳青霉烯类酶基因、AmpC酶基因、氨基糖苷类修饰酶基因、转座子遗传标记均阴性。本组菌株存在克隆传播现象。结论 自肺炎患儿分离的鲍曼不动杆菌连续分离株所携带的耐药基因的类型较成人少,且阳性率低,但存在克隆传播,应警惕医院内感染导致的暴发流行。
英文摘要:
      Abstract:Objective To investigate the drug resistance , genetic feature and genetic relationship of Acinetobacter baumannii(AB) isolated successively in pediatric patients with pneumonia. Methods 28 strains of AB were collected and isolated from the sputum culture of children with pneumonia, and were undertaken identification of bacteria and susceptibility test by Vitek-2 Compact automicroscan using GNI and GNS cards. Drug resistance genes ,integrons and transposons were analyzed by polymerase chain reaction (PCR). Results 4 of 28 strains of AB showed multidrug resistance, the positive rate was 14.29%. Beta-lactamase gene blaTEM, AmpC enzyme gene blaADC, modification gene of aminoglycosides aac(3)-II, aac(6’)-Ib, ant(3″)-I and aac(6″ )-II, integron genetic marker qacE△1-sul1, IntI1, and transposon genetic marker merA were all detected. Among them, 4 strains of blaTEM(14.29%),17 strains of blaADC(60.71%),1 strain of aac(3)-II(3.57%),1 strain of aac(6′)-Ib(3.57%),6 strains of ant(3″)-I(10.71%), 2 strains of aac(6″)-II(7.14%),11 strains of qacE△1-sul1(39.29%),4 strains of IntI1(14.29%)and 3 strains of merA(10.71%) were discovered,the rest of beta-lactamase gene, metallo beta-lactamase gene, carbopenems gene, AmpC gene, modification gene of aminoglycosides, transposon genetic markers showed negative. This indicated that clone transmission phenomenon was existing in this group. Conclusions Comparing with adults, The types of drug resistance genes carried in Acinetobacter baumannii(AB) isolated successively in pediatric patients were less, and positive rates were lower, but clone transmission phenomenon was existing in this group, we should be on guard against outbreak epidemic induced by nosocomial infection.
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