Abstract:Objective Preparation and using of Candida DNA probe. Methods The 125 hp DNA from E03 gene of C. albicans was amplified by PCR, then by hepen digoxigenin, and the 25 bp specific sequence from E03 gene was synthesized and labeled with biotin. The sensitivity of these probes was detected, and the specificity was detected by dot-blotting hybridization assay with 23 strains C. albicans isolated from patients, C. paropsilosis, C. tropicalis, C. krusei, C. stellatoidea and E. coli and so on. Results The Dig-125 hp DNA probe, only hybridized with C. albicans, whose sensitivity of color development was 0. 01 pg, and the hybridization result of the isolated strains was coincidental with the result of traditional identification for C. albicans. The Bio-25 hp DNA probe, hybridized with C. albicans, C. paropsilosis, C. tropicalis and C. stellatoidea, whose sensitivity of color development was 20 pg. Conclusion The Dig-125 bp DNA probe is specific and sensitive to C. albicans and suitable for the indentification of clinical C. albicans infection, while the Bio-25 bp DNA probe is suitable for the indentification of clinical candida infection.