Objective: To understand the incidence of candida vaginitis and the main types of Candida in this area,and explore the common antifungal antimicrobial sensitivity of C.albicans isolated from clinical. The ERG5 genes of C.albicans was amplified by PCR and sequencing and to investigate whether the mutation of ERG5 genes of C.albicans was associated with its drug resistance.Methods: During Jun to Dec 2018,vaginal secretions from gynecologic and obstetric outpatients were collected, and 285 strains of Candida were obtained by smear microscopy and CHROMagar chromogenic medium culture.Additionally, the identification and susceptibility tests of 156 C.albicans were detected by rapid fungal culture and drug sensitivity tests,as well as mass spectrometry. The ERG5 genes of C. albicans was amplified by PCR and sequencing for the analysis of mutation sites.Results: Of 285 fungi detected for the first time, C.albicans C.smooth, C.tropicalis, C.krusei and other fungi accounted for 156 (54.7%), 64(22.5%), 47(16.5%), 12(4.2%),and 6(2.1%),respectively. 156 strains of C.albicans were further identified by MALDI-TOF MS, indicating that One strain could mot be reliably identified(Score below 1.700) and the rest were C.albicans . strain Antimicrobial susceptibility tests in vitro showed that 155 strains of C.albicans were sensitive to 5-fluorouracil (5-FC), and the sensitivity to amphotericin (AMB) and nystatin (NYS) was 99.3% and 98.7%,respectively. However, the resistance rates of 155 strains against ketoconazole(KET),fluconazole(FIU),itraconazole(ITR),voriconazole(VOR),clotrimazole(CLO),miconazole(MIC), Econazole (ECO) were 43.59%, 62.82%, 61.54%, 50.64%, 50.64%, 51.92% and 51.28%, respectively. The ERG5 genes in 7 strains of C.albicans were chosen to be amplified by PCR and sequencing. Two mutation loci of ERG5 were found at the same site, one of which was synonymous mutation (G528A) and the other was missense mutation (G528C).Conclusions: (1)The proportion of C.albicans in VVC patients showed a downward trend, while non C.albicans gradually increased in our hospital.(2)When the traditional phenotypic identification is difficult to determine, it can be supplemented by mass spectrometry technique. (3)C.albicans is highly sensitive to 5-FC, AMB and NYS, but the resistance to azole drugs increased. There are cross-resistance and multi-drug resistance among C.albicans.(4)The mutation of ERG5 gene at 528 locus in C.albicans may be related to azole resistance.