Abstract: Objective To analyze the construction of platelet donor gene database in Changsha area, and evaluate the possbility of providing HLA/ HPA-matched apheresis platelets for the clinic. Methods The distributions of HPA and HLA-A/B alleles and antigens in 1 029 unrelated and random platelet donors in Changsha area were analyzed, and the construction scale of platelet donor gene database in Changsha area was evaluated. Results Without considering the ABO blood types, it was deduced that when the platelet donor database had a capacity of 207 persons, about 95% of patients could find at least one HPA genotype fully matched donor in the database. When the database had a capacity of 2 127 persons, about 95% of patients could find at least one HLA-A and -B phenotype fully matched donor in the database. When the database had a capacity of 1 029 persons, about 79.25% of patients could find at least one HLA-AB phenotype fully matched donor in the database. The highest mismatch probabilities of CREG epitopes of A and B antigens in apheresis platelet donors were 1C-2C and 7C-5C, respectively. The highest mismatch probability of Broad CREG was 4C-6C. Conclusion A platelet donor gene database with known HLA/HPA genotypes has been established in Changsha area, with a capacity of 1 029 persons. While expanding the efective capacity, the applicability of theoretical modeling and conformity of matching probability will be further verified.